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Effects of Soybean Isoflavones on Antioxidative Action in Aged Rats

目的:研究大豆異黃酮對D半乳糖致衰老大鼠抗氧化能力的影響。方法:用D半乳糖注射Wistar雄性大鼠5個月,建立衰老模型。對致衰老模型組、大豆異黃酮組肝臟、心臟和前列腺丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽過氧化酶(GSHPx)活性進行測定及比較。結果:低、中、高不同劑量大豆異黃酮灌喂組與模型組大鼠相比,各臟器MDA含量(μmol/L)(心臟:695±093,562±112,435±112比802±111;肝臟:815±085,647±120,515±112比935±135;前列腺:715±092,558±115,423±125比833±124)均有降低,差異有統計學意義(Plt;005),而SOD酶活性(nmol/L)(心臟:4732±308,5518±428,6120±368比3225±370;肝臟:18121±506,19015±706,19720±570比17213±512;前列腺:4156±301,4607±421,5015±335比3374±305)和GSHPx酶活性(nmol/L)(心臟:905±096,1111±245,1313±146比713±151;肝臟:902±105,1150±223,1362±192比698±160;前列腺:435±085,613±102,747±155比312±106)有升高,差異同樣具有統計學意義(Plt;005);大豆異黃酮攝入量越高,MDA含量越低,而SOD、GSHPx酶活性越高。結論:攝入適量大豆異黃酮可有效增強大鼠機體抗氧化能力,從而延緩D半乳糖誘發的大鼠衰老。

Analysis of Paraoxonase 1 Activity in the Patients with Hyperhyroidism

目的:探討甲狀腺功能亢進癥(甲亢)患者血漿對氧磷酯酶1(PON1)活性變化以及與其它氧化應激指標的關系。方法:分別測定50名對照組和78例甲亢組空腹血漿中游離三碘甲狀腺原氨酸(FT3)、游離甲狀腺素(FT4)、促甲狀腺激素(TSH)、PON1活性、超氧化物歧化酶(SOD)、丙二醛(MAD)、氧化低密度脂蛋白(ox-LDL)及血脂含量,并進行相關性分析。 結果:甲亢患者血漿PON1活性(139 ±64)kU/L,ox-LDL(598.3±58.6)μg/L,MDA(15.11±3.26) μmol/L及SOD(80.2±25.3)NU/mL。對照組上述指標分別為:PON1(168 ±70)kU/L,ox-LDL (446.2±62.2) μg/L,MDA (10.02±3.00) μmol/L,SOD(92.9±26.9)NU/mL。血漿PON1和SOD活性顯著低于對照組(Plt;0.01),ox-LDL和MDA水平顯著高于對照組(Plt;0.01)。甲亢患者血漿PON1活性與SOD呈正相關(r=0. 381,Plt; 0.05),與ox-LDL、MDA呈負相關(r=-0. 411,r=-0. 445,Plt; 0.01)。 結論:甲亢患者血漿PON1活性顯著降低,可能與氧化應激增強有關。

The Effect of Hypoxic Preconditioning on Old Rat Model of Postoperative Fatigue Syndrome Caused by Excision of Bilateral Accessory and Hysterectomy

目的:研究缺氧預處理對老年大鼠子宮及雙附件切除術后疲勞是否有改善作用,并通過對比觀察超氧化物歧化酶及丙二醛水平的變化,初步探討缺氧預處理的作用機制。方法:將老年大鼠分為空白對照組、對照組、缺氧預處理三組。空白對照組為假手術組,對照組為子宮及雙附件切除術組, 缺氧預處理組為缺氧預處理加子宮及雙附件切除術組。對比觀察缺氧預處理對大鼠體力活動及血清超氧化物歧化酶和丙二醛水平的影響。結果:空白對照組、對照組、缺氧預處理三組大鼠懸尾不動時間分別為:(21±3)s,(83±10)s,(44±5)s,各組間比較Plt;0.05。三組SOD活性分別為:(131.23±5.31)U/L,(36.12±9.68)U/L,(73.01±9.82)U/L,各組間比較Plt;0.05。三組MDA水平分別為:(9.78±1.26)μmol/L,(29.87±3.13)μmol/L,(15.98±2.21)μmol/L,各組間比較Plt;0.05。結論:缺氧預處理可提高老年大鼠的抗氧化能力,對老年大鼠子宮及雙附件切除術后疲勞綜合征有明顯的改善作用。

Influence of chronic alcohol intake on acute lung injury in rats

Objective To investigate the influence of chronic alcohol ingestion on the severity of acute lung injury (ALI) induced by oleic acid and lipopolysaccharide (LPS).Methods Thirty-two SD rats were randomly administrated with alcohol or water for 6 weeks，then instilled with oleic acid and LPS to induce ALI or with normal saline as control.Thus the rats were randomly divided into two injury groups [ethanol group and water group] and two control groups [ethanol group and water group] (n=8 in each group). PaO2,Wet to dry lung weight ratio (W/D)，levels of γ-glutamylcysteinylglycine (GSH) and malonaldehyde (MDA) in the lung tissue were measured.Results Compared to corresponding control groups，the PaO2 and GSH significantly decreased，and the lung W/D and MDA level were significantly increased in the injury groups (all Plt;0.05).In the injury groups，the changes of above parameters were more significant in the alcohol group than thoe in the water group (all Plt;0.05)，except the lung W/D with no significant difference.Conclusion Chronic ethanol ingestion was relevalent to oxidation/ antioxidation imbalance and more severe lung injury in rats with severe septic after trauma,which suggests that chronic alcohol abuse could increase the severity of acute lung injury.

Intermittent Hypoxic Preconditioning Alleviates Hepatic Ischemia Reperfusion Injury after Partial Hepatectomy in Rats by Antioxidant Pathways

目的探討間斷低氧預適應對大鼠肝大部切除術后殘余肝臟合并缺血再灌注引發過氧化損傷的保護作用。 方法78只SD大鼠，用SPSS軟件將其隨機分為4組：假手術組（SO組，n=6）、肝切除組（PH組，n=24）、肝切除合并缺血再灌注損傷組（IR組，n=24）和間斷低氧預適應組（IHP組，n=24）。以無創傷血管夾阻斷IR組大鼠入肝血流后切除肝臟的左葉和中葉（約占全肝的70%），20 min后開放入肝血流，殘余肝臟發生了缺血再灌注損傷。將IHP組大鼠暴露于10%的低氧環境中，每日持續1 h，連續進行1周，最后1次低氧暴露后行肝切除術（同IR組）。SO組大鼠在術后2 h取材檢測，其余各組分別于術后2、6、12及24 h進行檢測。檢測血清轉氨酶（ALT、AST）水平和肝勻漿組織中超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。 結果術后2 h，PH組、IR組和IHP組大鼠血清ALT和AST水平均高于SO組（P<0.05）。在術后6、12和24 h，IHP組大鼠血清ALT和AST均高于PH組，但低于IR組（均P<0.05）。與IR組相比，IHP組大鼠術后各時間點殘余肝臟中SOD活性明顯升高，而MDA含量則顯著降低（均P<0.05）。 結論間斷低氧預適應對殘余肝臟缺血再灌注損傷具有保護作用，其機理可能與提高肝臟的抗氧化能力有關。

The Effect of Rotenone on Dopaminic Neurons of Brain in Rat Model of Parkinson's Disease

ObjectiveTo study the effect of rotenone on rat substantia nigra dopamine (DA) in the nervous system and oxidative stress parameters (malondialdehyde and glutathione), the influence of rotenone on DA neurons toxic effect and its pathogenesis. MethodsThis study applied back subcutaneous injection of rotenone in rats [1.0 mg/(kg·d)], and used immunocytochemistry technique to detect changes in the expression of tyrosine kinase (TH) in 10 rats of the control group and 10 rats of the experimental group. Spectrophotometry was used to detect the change of oxidative stress parameters in rats (malondialdehyde and glutathione). ResultsDA neurons in rats had various degrees of damage. The TH immune response strength of rats in the substantia nigra and striatum decreased significantly. The number of immune response nigra TH positive neurons was significantly less in the experimental group than in the control group (P< 0.01). Spectrophotometer method was used to detect the midbrain nigra of glutathione, which was significantly less in the experimental group than in the control group (P<0.01). Malondialdehyde in the experimental group was significantly higher (P<0.01). ConclusionRotenone has obvious neurotoxicity, and can lead to the damage of DA neurons and obvious oxidative stress injury in rats, which provides an experimental basis for the pathogenesis of Parkinson's disease, and at the same time provides new targets for the treatment.

眼前段堿燒傷后視網膜病理改變及氧自由基反應

Protective Role of Recombinant Human Growth Hormone in Ischemic Reperfusion Injury of Rat Liver

【Abstract】　Objective　To investigate the protective role of recombinant human growth hormone (rhGH )in ischemic reperfusion injury of rat liver and its mechanism. Methods　One hundred Male rats were randomly divided into two groups: the rhGH group and the control group. In the rhGH group, rhGH were injected （0.2U/100g weight） to rats seven days before the ischemic reperfusion injury, and in the control group, normal saline was injected instead. Serum levels of ALT， TNF-α and IL-1α were tested. Hepatic tissue was sectioned for to detect the level of EC and MDA, the expression of NF-κB and ICAM-1 mRNA on SEC. Ultrastructural characteristics histopathological characteristics were determined also. Results　Serum levels of ALT, TNF-α, IL-1α and the contents of MDA in the control group were significantly higher than those in the rhGH group （P＜0.05）. Comparied with control group, rhGH also decreased NF-κB activation, and reduced the expression of ICAM-1 mRNA of SEC in the liver cells （P＜0.05）. Electronic microscopic revealed that the hepatic sinusoidal endothelial cells and the hepatocellular mitochondria were injured in the control group. Pretreatment with the rhGH was able to significantly improved the pathological changes. Conclusion　rhGH might confer the protection to ischemic reperfusion injury of rat liver through reducing the expression of NF-κB to down-regulate cytokine (IL-1α,TNF-α), MDA and inhibition the expression of ICAM-1 mRNA.

EXPERIMENTAL STUDY ON EFFECT OF REDUCED GLUTATHIONE ON RANDOM FLAP SURVIVAL IN RATS

Objective To investigate the effects of glutathione (GSH) on survival of the random skin flap in rats and the probable mechanism that contribute to this effect. Methods Twenty SD rats with 200-250 g in weight, were randomly divided into the experimental group and control group(n=10). Random flap of 8 cm×2 cm in size was made on the back of each rat with the pedicel on the angular of the scapular. GSH(250 mg/kg) and NS of the same dose were injected into the abdominal cavity of rats in the experimental groupand the control group immediately after the operative, 1st and 2nd days respectively. The rats were killed on the 7th day after the operation. The tissue pathology, the survival rate of the flap, the superoxide dismutase(SOD) activity and malonyldialdehyde (MDA) level were compared between two groups. Results The mean survival rate of the flap on the 7th day in the experimental group（56.77%±10.67%） was higher than that in the control group(40.16%±7.12%)(Plt;0.05).SOD activity in experimental group （306.06±84.87 U/mgprot）was higher than that in the control group （224.79±27.12 U/mgprot）, while MDA level （3.835±0.457 nmol/mgprot）was lower than that in the control group (6.127±0.837 nmol/mgprot)(Plt;0.05). Histological observation showed that the neutrophil infiltration was less in experimental group than that in the control group; that the experimental group was surperior to the control group in angiogenesis, fibroblasts, fair cells and cuaneous gland. Conclusion The intraperitoneal use of GSH may promote the survival rate of the random flaps and the possible mechanism for improvement may lies in that the GSH can reduce the level of oxygen free radical and lipidperoxidation,and lessen neutrophil infiltration.

Protective Effects of Epidermal Growth Factor on Pancreas of Rats with Acute Pancreatitis

【Abstract】Objective To investigate the protective effects of epidermal growth factor (EGF) on pancreas of rats with acute pancreatitis(AP). Methods Seventytwo male SpragueDawley rats were randomly divided into 3 groups: Control group, AP group and AP-EGF group. Subcutaneously injection of EGF (0.1 μg/g) were given to animals in the AP-EGF group after the establishment of the model of AP. The other two groups of animals received the same volume of saline. At 6 h, 12 h and 24 h after induction of AP, 8 animals in each group were sacrificed respectively， 4 ml of blood sample was withdrawn from heart，2 ml for the analysis of amylase activity and 2 ml for MDA content in serum. Ascites was sucked with dry gauzes and was weighed thereafter. Changes of pancreas morphology were evaluated at every time point. The same part of pancreas was removed for measurement of MDA content, apoptotic index (AI) and histologic changes. Results Histologic injury of the animals in the APEGF group was milder than that in the AP group. Ascites weight in the AP-EGF group decreased significantly compared with that in the AP group at 12 h and 24 h 〔(4.53±1.29) g vs (6.58±1.47) g, (7.64±1.85) g vs (11.96±2.13) g，P＜0.05，P＜0.01〕. Amylase activity in the APEGF group also decreased significantly compared with that in the AP group at 12 h and 24 h 〔(142.0±8.3) U/L vs (187.9±10.4) U/L, (194.3±10.4) U/L vs (253.3±8.6) U/L, P＜0.05，P＜0.01〕. MDA content in plasm 〔(2.34±0.23) μmol/L vs (3.15±0.38) μmol/L, P＜0.05〕 and in pancreas 〔(5.21±1.46) μmol/g vs (7.68±1.63) μmol/g, P＜0.01〕 in the APEGF group decreased significantly compared with those in the AP group at 24 h. AI of pancreas in the APEGF group increased significantly compared withthatintheAPgroupafteroperation〔(16.22±3.53)%〖KG4vs (7.35±1.04)%, (11.67±2.40)% vs (4.81±0.86)%, (6.38±1.42)% vs (1.97±0.21)%, P＜0.01〕. Conclusion EGF may accelerate the restoration of pathologic injury and alleviate the hemorrhage and edema of pancreas. It may also depress MDA content in plasm and in pancreas so that to lessen oxidative damage. EGF may protect pancreas by inducing cellular apoptosis.