【摘要】 目的 探討不同年齡段和不同內膜準備方法對凍融胚胎移植（FET）妊娠率的影響。 方法 回顧性分析2007年3月-2009年8月107例患者131個FET周期，比較不同年齡段、不同內膜準備方法的妊娠情況。 結果 ①lt;29歲、30～34歲、≥35歲患者的周期臨床妊娠率分別為45.28%、37.88%、33.33%，比較無統計學意義（Pgt;0.05）。②自然周期和激素替代周期內膜準備，兩種方法患者平均年齡、平均移植胚胎數、胚胎評分及胚胎細胞數比較無統計學意義（Pgt;0.05），但自然周期的胚胎種植率及周期臨床妊娠率均明顯低于激素替代周期（16.78%比29.21%；29.51%比50.00%），比較有統計學意義（Plt;0.05）。 結論 患者年齡不影響FET周期臨床妊娠率，激素替代周期內膜準備的FET胚胎種植率及周期臨床妊娠率明顯高于自然周期。【Abstract】 Objective To analyze the effect of age and method of preparing endometrium on the pregnancy rate of frozen-thawed embryo transfer (FET) among infertile women. Methods Retrospective analysis was made on 131 FET cycles in 107 patients from March 2007 to August 2009. The relationship between pregnancy rate of FET and following factors were analyzed: age, the method of preparing uterus endometrium. Results ①There was no significant difference in pregnancy rate of FET among ＜29, 30 - 34, and ≥35 years old patients (45.28%, 37.88%, and 33.33%, respectively) (Pgt;0.05). ②There was no significant difference in patients’ age, the average embryo amount, embryo score, the cell amount in every embryo between the FET with hormone replacement therapy (HRT) and the FET with natural cycle (NC). The embryo implantation rate and pregnancy rate of FET with NC were lower significantly than those of FET with HRT (16.78% vs 29.21%; 29.51% vs 50.00%) (P＜0.05). Conclusion Age might not influence pregnancy rate of FET. FET with HRT has higher implantation rate and pregnancy rate than FET with NC.
【摘要】 目的 觀察提前去除卵丘細胞對胚胎質量的影響，進一步探討其臨床應用價值。 方法 將2009年7-9月行體外受精-胚胎移植治療的患者隨機分為兩組，試驗組共137個周期，于授精后5 h去除卵丘細胞；對照組共146個周期，于授精后16～18 h去除卵丘細胞。兩組均于授精后16～18 h觀察受精情況，72 h對胚胎進行評分，選擇1～3枚優質胚胎進行胚胎移植。分別比較兩組的年齡、不育年限、獲卵數、受精率、卵裂率、優質胚胎率、胚胎種植率和臨床妊娠率。 結果 兩組的優質胚胎率差異有統計學意義（Plt;0.01）；但年齡、不育年限、獲卵數、受精率、卵裂率、胚胎種植率和臨床妊娠率比較，差異均無統計學意義（Pgt;0.05）。 結論 體外受精中提前去除卵丘細胞優質胚胎增加，有助于提高臨床累積妊娠率。【Abstract】 Objective To evaluate the effect of denuding the oocyte granulose cells soon after fertilization in vitro (IVF). Methods A total of 283 IVF cycles in patients from July to September 2009 were divided into a trial group and a control group randomly. The trial group contained 137 cycles, being denuded the oocyte granulose cells only 5 hours after IVF. The controlled group contained 146 cycles, being denuded the oocyte granulose cell and observed the pronuclear 18 hours after IVF. The outcomes of female age, infertile duration, the number of oocyte, fertilization rate,abnormal fertilization rate, good quality embryo rate, and clinical pregnancy rate were measured. Results There was no difference in any of the outcomes between the two groups except for the high quality embryo rate (Plt;0.01). The clinical pregnancy rate was higher in the trial group without significant differences (Pgt;0.05). Conclusions Denuding the oocyte granulose cells soon after IVF helps to improve the embryo quality and the clinical accumulated pregnancy rate.
