【摘要】 目的 探討細胞角蛋白19的可溶性片段CYFRA21-1在慢性腎臟病（chronic kidney disease，CKD）患者血清中的表達及其臨床意義。 方法 2008年10月-2009年4月隨機選取45例CKD患者，根據肌酐清除率（creatinine clearance rate，Ccr）的大小分為Ccrlt;15 mL/min組（23例）和Ccrgt;15 mL/min組（22例），并以正常體檢者（10例）為正常對照組，肺鱗癌患者（10例）為陽性對照組，每位參與研究者取血后采用酶聯免疫吸附法測定血清CYFRA21-1的濃度。 結果 正常對照組、陽性對照組、Ccrgt;15 mL/min組和Ccrlt;15 mL/min組的血清CFRA21-1濃度分別為（1.720±0.535）、（21.010±11.809）、（3.310±1.569）和（5.090±1.306） ng/mL。與正常對照組比較，其余3組的CYFRA21-1濃度均顯著升高，有統計學意義（Plt;0.05），且均值大于正常參考值（0~3.3 ng/mL）；Ccrgt;15 mL/min組和Ccrlt;15 mL/min組的CFRA21-1濃度顯著低于陽性對照組，有統計學意義（Plt;0.05）；兩個實驗組間隨著Ccr的降低，CYFRA21-1濃度升高，有統計學意義（Plt;0.05）。 結論 CKD患者血清CYFRA21-1水平的升高與腎功能減退存在一定關系，可將其作為臨床上預測腎臟纖維化程度的指標。【Abstract】 Objective To discuss the clinical Between significance of cytokeratin 19 fragment (CYFRA21-1) in patients with chronic kidney disease (CKD). Methods From October 2008 to April 2009, 45 inpatients were randomly selected and assigned into three groups according to creatinine clearance rate (Ccr) level: Ccrlt;15 mL/min, Ccrgt;15 mL/min. Ten healthy volunteers were chosen as control group, and other 10 patients with lung squamous cell carcinoma as positive control group. ELISA was used to measure the serum concentration of CYFRA21-1. Results Compared with control group, the serum concentration of CYFRA21-1 in CKD groups and positive control group was elevated (Plt;0.05). As the Ccr decreased, the serum concentration of CYFRA21-1 was elevated (Plt;0.05) in two CKD groups. Conclusion Serum concentration of CYFRA21-1 in CKD patients has a relationship with the renal function decrease, and may be used as an indication of renal interstitial fibrosis (RIF).
