Epstein-Barr (EB) virus infection is associated with various tumors of lymphoid and epithelial origin. EB virus exists in most humans as a latent infection. EB virus latent infection-related genes play a key role in the EB virus latent infection, and also play an important role in promoting the occurrence and development of related tumors. This article will briefly introduce the characteristics of EB virus latent infection, the protein coding genes and non-coding genes related to EB virus latent infection (including EB virus nuclear antigen genes, EB virus latent membrane protein genes, EB virus encoded small RNA genes and EB virus microRNA genes), and the main functional mechanism of these EB virus latent infection-related genes in EB virus latent infection and subsequent tumorigenesis. The purpose is to providea theoretical basis for a comprehensive understanding of the EB virus latent infection and the mechanism of tumors caused by EB virus.
Human immunodeficiency virus (HIV)-1 p24 antigen is one of the earliest proteins appearing after HIV infection. It can be used as a diagnostic marker to shorten the detection “window period” to about 14 days. It is of vital importance in the process of early diagnosis and antiviral therapy monitoring. This review briefly describes the basic structure and clinical significance of detection of HIV-1 p24 antigen, focusing on the current domestic and foreign researches on the biosensors of HIV-1 p24 antigen based on new nanomaterials, in order to provide a reference for developing novel detection technology.
Medical engineering interdisciplinary aims to integrate medicine, engineering science and other disciplines and to carry out scientific research and application in a collaborative manner. At present, the demand for medical engineering interdisciplinary in the field of medical laboratory technology is increasing, which also puts forward new requirements for the “Clinical Microbiology Laboratory Technology”. This article elaborates on the necessity and existing problems of the medical engineering interdisciplinary, reports on the overall ideas of the curriculum reform of “Clinical Microbiology Laboratory Technology” at West China School of Medicine of Sichuan University based on the medical engineering interdisciplinary, and specifically elaborates on the reform practices in three aspects: teaching philosophy, teaching content and methods, and teacher optimization, as well as the preliminary achievements obtained, so as to provide new ideas for the curriculum reform of the “Clinical Microbiology Laboratory Technology”.
The teaching team of the Department of Laboratory Medicine, West China School of Medicine, Sichuan University takes scientific spirit, craftsmanship spirit, and medical humanities spirit as the goal, combines the core knowledge of clinical microbiology laboratory technology course with cases of coronavirus disease 2019 prevention and control, drug resistance monitoring, and scientists’ deeds, and adopts strategies such as hot debate and special report to achieve the integration of knowledge and value. The actual effectiveness of this model has been verified through the assessment of students’ abilities and values. This article introduces the course-based ideological and political education construction mentioned above, aiming to explore the integration path of course-based ideological and political education in the clinical microbiology laboratory technology, and construct a new teaching model that combines professional competence and value guidance.
Objective To evaluate the performance of a novel magnetic particle-based chemiluminescence reagent for detecting cryptococcal capsular polysaccharide. Methods Serum and cerebrospinal fluid specimens were collected from patients with suspected cryptococcal infection at West China Hospital, Sichuan University between July 2022 and September 2023. All samples were simultaneously tested by chemiluminescence immunoassay (CLIA) and lateral flow immunoassay (LFA), and the results of the two methods were compared. Results A total of 357 patients with suspected cryptococcal infection were enrolled, including 247 serum samples and 110 cerebrospinal fluid samples. The novel magnetic particle-based CLIA reagent exhibited favorable clinical performance for cryptococcal infection detection. For serum and cerebrospinal fluid specimens, the sensitivity was 96.95% and 100.00%, respectively; the specificity was 99.14% and 100.00%, respectively. It showed high consistency with the LFA reagent (the kappa values were both greater than 0.98 for serum and cerebrospinal fluid samples). Moreover, the CLIA reagent enables quantitative measurement for clinical therapeutic monitoring. Conclusion The novel magnetic particle-based CLIA reagent possesses good diagnostic performance and can provide reliable and supportive laboratory evidence for clinical practice in both early diagnosis and therapeutic monitoring.
