LIYachai 1 , HUANGXianghua 2 , ZHANGMingle 2 , LIYanan 2 , CHENYexing 3 , JIAJingfei 3
  • 1. Department of Obstetrics and Gynecology, Affiliated Hospital of Hebei University, Baoding Hebei, 071000, P. R. China;
  • 2. Department of Obstetrics and Gynecology, Second Hospital of Hebei Medical University;
  • 3. Department of Obstetrics and Gynecology, Fuping Hospital;
HUANGXianghua, Email: huangxh2003@163.com
Tissue engineered vagina; Poly-lactide-co-glycolide; Collagen type I; Vaginal epithelial cells; Biocompatibility
10.7507/1002-1892.20150248
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Objective To explore the biocompatibility of the poly-lactide-co-glycolide (PLGA)/collagen type I scaffold with rat vaginal epithelial cells, and the feasibility of using PLGA/collagen type I as scaffold to reconstruct vagina by the tissue engineering. Methods PLGA/collagen type I scaffold was prepared with PLGA covered polylysine and collagen type I. The vaginal epithelial cells of Sprague Dawley rat of 10-12 weeks old were cultured by enzyme digestion method. The vaginal epithelial cells of passage 2 were cultured in the leaching liquor of scaffold for 48 hours to detect its cytotoxicity by MTT. The vaginal epithelial cells were inoculated on the PLGA/collagen type I scaffold (experimental group) and PLGA scaffold (control group) to calculate the cell adhesion rate. Epithelial cells-scaffold complexes were implanted subcutaneously on the rat back. At 2, 4, and 8 weeks after implantation, the epithelial cells-scaffold complexes were harvested to observe the cell growth by HE staining and immunohistochemical analysis. The epithelial cells-scaffold complexes were transplanted to reconstruct vagina in 6 rats with vaginal defect. After 3 and 6 months, the vaginal length was measured and the appearance was observed. The neovagina tissues were harvested for histological evaluation after 6 months. Results The epithelial cells grew and proliferated well in the leaching liquor of PLGA/collagen type I scaffold, and the cytotoxicity was at grade 1. The cell adhesion rate on the PLGA/collagen type I scaffold was 71.8%±9.2%, which significantly higher than that on the PLGA scaffold (63.4%±5.7%) (t=2.195, P=0.005). The epithelial cells could grow and adhere to the PLGA/collagen type I scaffolds. At 2 weeks after implanted subcutaneously, the epithelial cells grew and proliferated in the pores of scaffolds, and the fibroblasts were observed. At 4 weeks, 1-3 layers epithelium formed on the surface of scaffold. At 8 weeks, the epithelial cells increased and arranged regularly, which formed the membrane-like layer on the scaffold. The keratin expression of the epithelium was positive. At 3 months after transplantation in situ, the vaginal mucosa showed pink and lustrous epithelialization, and the majority of scaffold degraded. After 6 months, the neovagina length was 1.2 cm, without obvious stenosis; the vaginal mucosa had similar appearance and epithelial layer to normal vagina, but it had less duplicature; there were nail-like processes in the basal layer, but the number was less than that of normal vagina. The immunohistochemistry staining for keratin was positive. Conclusion The PLGA/collagen type I scaffolds have good cytocompatibility with the epithelial cells, and can be used as the biodegradable polymer scaffold of the vaginal tissue engineering.

Citation： LIYachai, HUANGXianghua, ZHANGMingle, LIYanan, CHENYexing, JIAJingfei. BIOCOMPATIBILITY OF POLY-LACTIDE-CO-GLYCOLIDE /COLLAGEN TYPE I SCAFFOLD WITH RAT VAGINAL EPITHELIAL CELLS. Chinese Journal of Reparative and Reconstructive Surgery, 2015, 29(9): 1144-1149. doi: 10.7507/1002-1892.20150248 Copy

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